Changes in chemical properties and microbial communities’ composition of a forest litter–based biofertilizer produced through aerated solid-state culture under different oxygen conditions - Biotechnologie Environnementale et Chimiométrie Accéder directement au contenu
Article Dans Une Revue Environmental Science and Pollution Research Année : 2024

Changes in chemical properties and microbial communities’ composition of a forest litter–based biofertilizer produced through aerated solid-state culture under different oxygen conditions

Résumé

Fermented forest litter (FFL) is a bioproduct used as biofertilizer for several decades in Eastern Asia and Latin America. It is locally handcrafted by farmers in anaerobic conditions by fermenting forest litter added with agricultural by-products such as whey, cereal bran, and molasses. The aim of this study was to characterize the FFL process and product through gas and liquid chromatography analyses. It also provides some highlights on the influence of O2 on this solid-state culture. Under anoxic condition, a maximum CO2 production rate (CDPR) of 0.41 mL/h∙g dry matter (dm) was reached after 8 days. The main volatile organic compounds (VOCs) were ethanol and ethyl acetate, with a production rate profile similar to CDPR. After 21 days of culture, no residual sucrose nor lactose was detected. Lactic and acetic acids reached 58.8 mg/g dm and 10.2 mg/g dm, respectively, ensuring the acidification of the matrix to a final pH of 4.72. A metabarcoding analysis revealed that heterolactic acid bacteria (Lentilactobacillus, Leuconostoc), homolactic acid bacteria (Lactococcus), and yeasts (Saccharomyces, Clavispora) were predominant. Predicted genes in the microbiome confirmed the potential link between detected bacteria and acids and VOCs produced. When O2 was fed to the cultures, final pH reached values up to 8.5. No significant amounts of lactic nor acetic acid were found. In addition, a strong shift in microbial communities was observed, with a predominance of Proteobacteria and molds, among which are potential pathogens like Fusarium species. This suggests that particular care must be brought to maintain anoxic conditions throughout the process.
Fichier sous embargo
Fichier sous embargo
0 11 3
Année Mois Jours
Avant la publication
vendredi 30 mai 2025
Fichier sous embargo
vendredi 30 mai 2025
Connectez-vous pour demander l'accès au fichier

Dates et versions

ird-04579418 , version 1 (30-05-2024)

Identifiants

Citer

L Miche, Alizée Dries, Inès Ben Ammar, Sylvain Davidson, Loris Cagnacci, et al.. Changes in chemical properties and microbial communities’ composition of a forest litter–based biofertilizer produced through aerated solid-state culture under different oxygen conditions. Environmental Science and Pollution Research, 2024, ⟨10.1007/s11356-024-33629-8⟩. ⟨ird-04579418⟩
27 Consultations
1 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More